prikaz prve stranice dokumenta Effects of Vanadate on Glucose Production in Cultured Hepatocytes Isolated from Rats on High Saturated Fat Diet
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Scientific paper - Original scientific paper
Effects of Vanadate on Glucose Production in Cultured Hepatocytes Isolated from Rats on High Saturated Fat Diet
Collegium antropologicum, 29 (2005), 2; 693-696. urn:nbn:hr:220:460983
Roša, Jagoda; Skala, Hana; Roša, Josip

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Roša, J., Skala, H. & Roša, J. (2005). Effects of Vanadate on Glucose Production in Cultured Hepatocytes Isolated from Rats on High Saturated Fat Diet. Collegium antropologicum, 29. (2), 693-696. Retrieved from https://urn.nsk.hr/urn:nbn:hr:220:460983

Roša, Jagoda, et al. "Effects of Vanadate on Glucose Production in Cultured Hepatocytes Isolated from Rats on High Saturated Fat Diet." Collegium antropologicum, vol. 29, no. 2, 2005, pp. 693-696. https://urn.nsk.hr/urn:nbn:hr:220:460983

Roša, Jagoda, Hana Skala and Josip Roša. "Effects of Vanadate on Glucose Production in Cultured Hepatocytes Isolated from Rats on High Saturated Fat Diet." Collegium antropologicum 29, no. 2 (2005): 693-696. https://urn.nsk.hr/urn:nbn:hr:220:460983

Roša, J., Skala, H. and Roša, J. (2005) 'Effects of Vanadate on Glucose Production in Cultured Hepatocytes Isolated from Rats on High Saturated Fat Diet', Collegium antropologicum, 29(2), pp. 693-696. Available at: https://urn.nsk.hr/urn:nbn:hr:220:460983 (Accessed 10 May 2024)

Roša J, Skala H, Roša J. Effects of Vanadate on Glucose Production in Cultured Hepatocytes Isolated from Rats on High Saturated Fat Diet. Collegium antropologicum [Internet]. 2005 [cited 2024 May 10];29(2):693-696. Available at: https://urn.nsk.hr/urn:nbn:hr:220:460983

J. Roša, H. Skala and J. Roša, "Effects of Vanadate on Glucose Production in Cultured Hepatocytes Isolated from Rats on High Saturated Fat Diet", Collegium antropologicum, vol. 29, no. 2, pp. 693-696, 2005. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:220:460983. [Accessed: 10 May 2024]

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Metadata
Title (english)Effects of Vanadate on Glucose Production in Cultured Hepatocytes Isolated from Rats on High Saturated Fat Diet
Title (croatian)Učinci vanadata na stvaranje glukoze u kultiviranim hepatocitima izoliranim u štakora na dijeti bogatoj zasićenim mastima
AuthorJagoda Roša
AuthorHana Skala
AuthorJosip Roša
Author's institution(Sestre milosrdnice University Hospital Center)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Internal Medicine
Abstract (english)
Insulin resistance is a common phenomenon in obesity and Type 2 diabetes. Common factor important for development of diabetes and insulin resistance is intake of saturated fat. Vanadate treatment improves glucose homeostasis in vivo. The aim of this study was to find out changing of hepatic glucose output in dependence of saturated fat diet and possible direct action of vanadate in cultured hepatocytes. Hepatocytes were isolated by a collagenase perfusion technique and cultured for 24 h in M 199 serum-free medium. The glucose production in hepatocytes isolated from rats on high saturated fat diet was significantly 139% higher comparable to standard controls. Glucagon 100% increased glucose production in hepatocytes from rats on standard diet and 200% in hepatocytes on saturated high fat diet. The addition vanadate significantly decreased basic glucose production and did not influence glucagon stimulated glucose production. Presence of insulin did not influence either glucagon or vanadate effect. High saturated fat diet not only increases insulin resistance but also decreases chances of successful therapy of diabetes.
Abstract (croatian)
Inzulinska rezistencija je zajednički fenomen koji se pojavljuje u pretilosti i dijabetesu tipa 2. Zajednički faktor koji je važan za razvoj dijabetesa i inzulinske rezistencije je uzimanje zasićenih masti. Vanadate poboljšava glukoznu homeostazu in vivo. Svrha ovog rada je otkriti promjene jetrenog stvaranja i otpuštanja glukoze u ovisnosti o dijeti bogatoj zasićenim mastima i moguće direktno djelovanje vanadate u kultiviranim hepatocitima. Hepatociti su izolirani pomoću kolagenaze i kultivirani 24 sata u M 199 mediju bez dodatka seruma. Stvaranje glukoze u hepatocitima izoliranim iz štakora na visoko masnoj dijeti bogatoj zasićenim masnoćama bilo je 139% veća u usporedbi s standardnom kontrolom. Glukagon 100% povećava proizvodnju glukoze u hepatocitima dobivenim iz štakora na standardnoj dijeti, a 200% u štakora na dijeti sa zasićenim masnoćama. Vanadate je signifikantno smanjio bazičnu proizvodnju glukoze, a nije utjecao na glukagonom stimuliranu produkciju glukoze. Prisutnost inzulina nije utjecala na glukagonski kao ni na vanadate efekt. Dijeta bogata zasićenim masnoćama ne povećava samo inzulinsku rezistenciju nego također smanjuje izglede za uspješno liječenje dijabetesa.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCollegium antropologicum
Numberingvol. 29, no. 2, pp. 693-696
p-ISSN0350-6134
e-ISSN1848-9486
URN:NBNurn:nbn:hr:220:460983
Publication2005
Document URLhttps://hrcak.srce.hr/5334
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2023-08-23 09:51:39