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Scientific paper - Original scientific paper
Comparison of Two Different Methods for Tigecycline Susceptibility Testing in Acinetobacter Baumannii
Acta clinica Croatica, 57 (2018), 4; 618-623. https://doi.org/10.20471/acc.2018.57.04.02
Bedenić, Branka; Cavrić, Gordana; Vranić-Ladavac, Mirna; Barišić, Nada; Karčić, Natalie; Tot, Tatjana; Presečki-Stanko, Aleksandra; Lukić-Grlić, Amarela; Frančula-Zaninović, Sonja; Sreter, Katherina Bernadette

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Bedenić, B., Cavrić, G., Vranić Ladavac, M., Barišić, N., Karčić, N., Tot, T. ... Sreter, K. B. (2018). Comparison of Two Different Methods for Tigecycline Susceptibility Testing in Acinetobacter Baumannii. Acta clinica Croatica, 57. (4), 618-623. doi: 10.20471/acc.2018.57.04.02

Bedenić, Branka, et al. "Comparison of Two Different Methods for Tigecycline Susceptibility Testing in Acinetobacter Baumannii." Acta clinica Croatica, vol. 57, no. 4, 2018, pp. 618-623. https://doi.org/10.20471/acc.2018.57.04.02

Bedenić, Branka, Gordana Cavrić, Mirna Vranić Ladavac, Nada Barišić, Natalie Karčić, Tatjana Tot, Aleksandra Presečki Stanko, Amarela Lukić Grlić, Sonja Frančula Zaninović and Katherina Bernadette Sreter. "Comparison of Two Different Methods for Tigecycline Susceptibility Testing in Acinetobacter Baumannii." Acta clinica Croatica 57, no. 4 (2018): 618-623. https://doi.org/10.20471/acc.2018.57.04.02

Bedenić, B., et al. (2018) 'Comparison of Two Different Methods for Tigecycline Susceptibility Testing in Acinetobacter Baumannii', Acta clinica Croatica, 57(4), pp. 618-623. doi: 10.20471/acc.2018.57.04.02

Bedenić B, Cavrić G, Vranić Ladavac M, Barišić N, Karčić N, Tot T, and sur.. Comparison of Two Different Methods for Tigecycline Susceptibility Testing in Acinetobacter Baumannii. Acta clinica Croatica [Internet]. 2018 [cited 2024 December 16];57(4):618-623. doi: 10.20471/acc.2018.57.04.02

B. Bedenić, et al., "Comparison of Two Different Methods for Tigecycline Susceptibility Testing in Acinetobacter Baumannii", Acta clinica Croatica, vol. 57, no. 4, pp. 618-623, 2018. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:220:892149. [Accessed: 16 December 2024]

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Title (english)Comparison of Two Different Methods for Tigecycline Susceptibility Testing in Acinetobacter Baumannii
Title (croatian)Usporedba dviju različitih metoda za testiranje osjetljivosti na tigeciklin u Acinetobacter baumannii
AuthorBranka Bedenić
AuthorGordana Cavrić
AuthorMirna Vranić-Ladavac
AuthorNada Barišić
AuthorNatalie Karčić
AuthorTatjana Tot
AuthorAleksandra Presečki-Stanko
AuthorAmarela Lukić-Grlić
AuthorSonja Frančula-Zaninović
AuthorKatherina Bernadette Sreter
Author's institution(Sestre milosrdnice University Hospital Center)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Tigecycline susceptibility testing (TST) presents a tremendous challenge for clinical microbiologists. Previous studies have shown that the Epsilometer test (E-test) and Vitek 2 automated system significantly overestimate the minimum inhibitory concentrations for tigecycline resistance compared to the broth microdilution method (BMM). This leads to very major errors or false susceptibility (i.e. the isolate is called susceptible when it is actually resistant). The aim of this study was to compare E-test against BMM for TST in carbapenem-resistant and carbapenem-susceptible Acinetobacter (A.) baumannii and to analyze changes in tigecycline susceptibility between two time periods (2009-2012 and 2013-2014), with BMM as the gold standard. Using the EUCAST criteria, the rate of resistance to tigecycline for the OXA-23 MBL-positive, OXA-23 MBL-negative and carbapenemase-negative strains for BMM was 54.5% (6/11), 29.4% (5/17) and 2.7% (1/37), respectively; the OXA-24/40 and OXA-58 producing organisms did not exhibit any resistance. With E-test, all OXA-23 MBL-positive organisms (11/11), 23.5% (4/17) of OXA-23 MBL-negative, and 4.1% of OXA-24/40 (3/74) strains displayed tigecycline resistance; there were no resistant strains among the OXA-58 and carbapenemase-negative isolates. Resistance emerged in the bacterial isolates from 2013 to 2014. Although tigecycline does not display cross-resistance, the highest rates of resistant A. baumannii isolates were observed among those producing VIM MBL, regardless of the testing method. These findings suggest that the commercial E-test does not provide reliable results for TST of A. baumannii. Further confirmation with the dilution method should be recommended, particularly in cases of serious infections.
Abstract (croatian)
Testiranje osjetljivosti na tigeciklin (TST) je velik izazov za kliničke mikrobiologe. Prethodna istraživanja su pokazala da E-test i Vitek 2 daju veće vrijednosti minimalne inhibitorne koncentracije tigeciklina u odnosu na dilucijsku metodu, što uzrokuje vrlo veliku grešku (engl. very major error, što znači da je rezistentan izolat proglašen osjetljivim). Cilj istraživanja bio je usporediti dvije metode za testiranje osjetljivosti na tigeciklin (E-test i bujonska dilucijska metoda) u karbapenem osjetljvim i karbapenem rezistentnim izolatima Acinetobacter (A.) baumannii s različitim tipovima karbapenem-hidrolizirajućih oksacilinazama i analizirati promjenu u stopama osjetljivosti na tigeciklin u dva razdoblja istraživanja (2009.-2012. i 2013.-2014.). Dilucija u bujonu je bila referentna metoda. Testiranje osjetljivosti na tigeciklin je provedeno E-testom i bujonskom mikrodilucijskom metodom. Prema kriterijima EUCAST-a stopa rezistencije bila je 54,5% (6/11) za OXA-23 MBL-pozitivne sojeve, 29,4% (5/17) za OXA-23 MBL-negativne sojeve i 2,7% (1/37) za karbapenemaza-negativne sojeve uz bujonsku mikrodilucijsku metodu. OXA-24/40 i OXA-58 producirajući sojevi nisu iskazivali rezistenciju. E-testom su svi OXA-23 MBL pozitivni organizmi (11/11), 23,5% (4/17) OXA-23 MBL negativnih i 4,1% OXA-24/40 (3/74) pokazivali rezistenciju na tigeciklin. Svi OXA-58 pozitivni i karbapenemaza-negativni sojevi su bili osjetljivi na tigeciklin u E-testu. Rezistencija na tigeciklin se pojavila u razdoblju od 2013. do 2014. godine. Iako tigeciklin ne pokazuje križnu rezistenciju s drugim antibioticima najviše stope rezistencije su zapažene među VIM-pozitivnim izolatima bez obzira na metodu testiranja. Prema rezultatima našega istraživanja komercijalni E-test ne daje pouzdane rezultate TST u A. baumannii. Potrebna je potvrda dilucijskom metodom, osobito kod teških infekcija.
Keywords (english)
Keywords (croatian)
Languageenglish
Languagecroatian
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleActa clinica Croatica
Numberingvol. 57, no. 4, pp. 618-623
p-ISSN0353-9466
e-ISSN1333-9451
DOIhttps://doi.org/10.20471/acc.2018.57.04.02
URN:NBNurn:nbn:hr:220:892149
Publication2018
Document URLhttps://hrcak.srce.hr/217797
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-09-20 08:55:45