prikaz prve stranice dokumenta False Positive Phenotypic Detection of Metallo-Beta-Lactamases in Acinetobacter Baumannii
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Scientific paper - Original scientific paper
False Positive Phenotypic Detection of Metallo-Beta-Lactamases in Acinetobacter Baumannii
Acta clinica Croatica, 58 (2019), 1; 113-118. https://doi.org/10.20471/acc.2019.58.01.15
Bedenić, Branka; Ladavac, Ranko; Vranić-Ladavac, Mirna; Barišić, Nada; Karčić, Natalie; Sreter, Katherina Bernadette; Mihaljević, Slobodan; Bielen, Luka; Car, Haris; Beader, Nataša

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Bedenić, B., Ladavac, R., Vranić Ladavac, M., Barišić, N., Karčić, N., Sreter, K. B. ... Beader, N. (2019). False Positive Phenotypic Detection of Metallo-Beta-Lactamases in Acinetobacter Baumannii. Acta clinica Croatica, 58. (1), 113-118. doi: 10.20471/acc.2019.58.01.15

Bedenić, Branka, et al. "False Positive Phenotypic Detection of Metallo-Beta-Lactamases in Acinetobacter Baumannii." Acta clinica Croatica, vol. 58, no. 1, 2019, pp. 113-118. https://doi.org/10.20471/acc.2019.58.01.15

Bedenić, Branka, Ranko Ladavac, Mirna Vranić Ladavac, Nada Barišić, Natalie Karčić, Katherina Bernadette Sreter, Slobodan Mihaljević, Luka Bielen, Haris Car and Nataša Beader. "False Positive Phenotypic Detection of Metallo-Beta-Lactamases in Acinetobacter Baumannii." Acta clinica Croatica 58, no. 1 (2019): 113-118. https://doi.org/10.20471/acc.2019.58.01.15

Bedenić, B., et al. (2019) 'False Positive Phenotypic Detection of Metallo-Beta-Lactamases in Acinetobacter Baumannii', Acta clinica Croatica, 58(1), pp. 113-118. doi: 10.20471/acc.2019.58.01.15

Bedenić B, Ladavac R, Vranić Ladavac M, Barišić N, Karčić N, Sreter KB, and sur.. False Positive Phenotypic Detection of Metallo-Beta-Lactamases in Acinetobacter Baumannii. Acta clinica Croatica [Internet]. 2019 [cited 2024 December 09];58(1):113-118. doi: 10.20471/acc.2019.58.01.15

B. Bedenić, et al., "False Positive Phenotypic Detection of Metallo-Beta-Lactamases in Acinetobacter Baumannii", Acta clinica Croatica, vol. 58, no. 1, pp. 113-118, 2019. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:220:377071. [Accessed: 09 December 2024]

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Metadata
Title (english)False Positive Phenotypic Detection of Metallo-Beta-Lactamases in Acinetobacter Baumannii
Title (croatian)Lažno pozitivna fenotipska detekcija metalo-beta-laktamaza u Acinetobacter Baumannii
AuthorBranka Bedenić
AuthorRanko Ladavac
AuthorMirna Vranić-Ladavac
AuthorNada Barišić
AuthorNatalie Karčić
AuthorKatherina Bernadette Sreter
AuthorSlobodan Mihaljević
AuthorLuka Bielen
AuthorHaris Car
AuthorNataša Beader
Author's institution(Sestre milosrdnice University Hospital Center)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Phenotypic detection of metallo-β-lactamases (MBLs) in Acinetobacter (A.) baumannii is a serious challenge to clinical microbiologists. MBLs are inhibited by metal chelators such as ethylenediaminetetraacetic acid) (EDTA). Production of MBLs cannot be recognized based on resistance phenotype. Therefore, phenotypic tests using EDTA are recommended. The aim of this study was to investigate the sensitivity and specificity of inhibitor based tests (EDTA) for detection of MBL. A total of 172 A. baumannii strains (123 carbapenemase positive and 49 carbapenemase negative) were analyzed. Phenotypic detection of MBLs was performed by the combined disk test with EDTA (CDT-EDTA) and EPI-dilution test (EPI-DT). Both tests were positive in all 11 isolates possessing VIM-1 MBL, showing 100% sensitivity. However, false positive results were observed in strains with class D carbapenemases using both tests, i.e. all OXA-23 and OXA-24/40 producing organisms and most OXA-58 positive strains (77% with CDT-EDTA vs. 65% with EPI-DT). False positive results can occur because oxacillinases are converted to a less active state in the presence of EDTA, leading to augmentation of the inhibition zone around the carbapenem disk or reduction of carbapenem minimum inhibitory concentrations. This study showed high sensitivity but low specificity of phenotypic methods in the detection of MBLs.
Abstract (croatian)
Fenotipska detekcija metalo-β-laktamaza (MBL) u Acinetobacter (A.) baumannii je velik izazov kliničkim mikrobiolozima. MBL su inhibirane metalnim kelatorima kao što je etilendiamin tetraoctena kiselina (EDTA). Produkcija MBL ne može se prepoznati na temelju fenotipa rezistencije. Zbog toga se preporuča primjena fenotipskih testova koji rabe metalne kelatore kao što je EDTA. Cilj istraživanja bio je utvrditi osjetljivost i specifičnost testova s inhibitorom (EDTA) u otkrivanju MBL u A. baumannii. Analizirana su 172 izolata A. baumannii (123 karbapenemaza pozitivnih i 49 karbapenemaza negativnih). Fenotipska detekcija MBL je provedena metodom kombiniranih diskova (EDTA CDT-EDTA) i EPI-dilucijskom metodom (EPI-DT). Oba testa su bila pozitivna u svih 11 izolata pozitivnih na VIM-1 MBL, što odgovara osjetljivosti od 100%. Lažno pozitivni rezultati su dobiveni u karbapenem-rezistentnim izolatima pozitivnim na klasu D karbapenemaza u oba testa: svi OXA-23 i OXA-24/40 kao i većina OXA-58 pozitivnih sojeva (77% u CDT-EDTA i 65% u EPI-DT). Lažno pozitivni rezultati u testovima s inhibitorom nastaju zbog toga što oksacilinaze prelaze u stanje manje aktivnosti u prisustvu EDTA, što dovodi do smanjenja minimalne inhibitorne koncentracije karbapenema ili do uvećanja zone oko diska karbapenema. Studija je pokazala visoku osjetljivost, ali nisku specifičnost fenotipskih testova za dokaz MBL u A. baumannii, što ukazuje na neophodnost korištenja molekularnih metoda.
Keywords (english)
Keywords (croatian)
Languageenglish
Languagecroatian
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleActa clinica Croatica
Numberingvol. 58, no. 1, pp. 113-118
p-ISSN0353-9466
e-ISSN1333-9451
DOIhttps://doi.org/10.20471/acc.2019.58.01.15
URN:NBNurn:nbn:hr:220:377071
Publication2019
Document URLhttps://hrcak.srce.hr/220887
Type of resourceText
Access conditionsOpen access
Terms of useLicence In copyright icon
Created on2024-09-20 10:29:06