prikaz prve stranice dokumenta Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation
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Scientific paper - Original scientific paper
Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation
The International Journal of Developmental Biology, 65 (2021), 10-11-12; 537-543. https://doi.org/10.1387/ijdb.210115fs
Šepac, Ana; Bošnjak, Željko J.; Seiwerth, Sven; Sikirić, Sunčana; Regović Džombeta, Tihana; Kulić, Ana; Marunica Karšaj, Jelena; Sedlić, Filip

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Šepac, A., Bošnjak, Ž. J., Seiwerth, S., Sikirić, S., Regović Džombeta, T., Kulić, A. ... Sedlić, F. (2021). Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation. The International Journal of Developmental Biology, 65. (10-11-12), 537-543. doi: 10.1387/ijdb.210115fs

Šepac, Ana, et al. "Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation." The International Journal of Developmental Biology, vol. 65, no. 10-11-12, 2021, pp. 537-543. https://doi.org/10.1387/ijdb.210115fs

Šepac, Ana, Željko J. Bošnjak, Sven Seiwerth, Sunčana Sikirić, Tihana Regović Džombeta, Ana Kulić, Jelena Marunica Karšaj and Filip Sedlić. "Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation." The International Journal of Developmental Biology 65, no. 10-11-12 (2021): 537-543. https://doi.org/10.1387/ijdb.210115fs

Šepac, A., et al. (2021) 'Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation', The International Journal of Developmental Biology, 65(10-11-12), pp. 537-543. doi: 10.1387/ijdb.210115fs

Šepac A, Bošnjak ŽJ, Seiwerth S, Sikirić S, Regović Džombeta T, Kulić A, and sur.. Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation. The International Journal of Developmental Biology [Internet]. 2021 [cited 2024 December 28];65(10-11-12):537-543. doi: 10.1387/ijdb.210115fs

A. Šepac, et al., "Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation", The International Journal of Developmental Biology, vol. 65, no. 10-11-12, pp. 537-543, 2021. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:220:488644. [Accessed: 28 December 2024]

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Metadata
Title (english)Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation
AuthorAna Šepac
AuthorŽeljko J. Bošnjak
AuthorSven Seiwerth
AuthorSunčana Sikirić
AuthorTihana Regović Džombeta
AuthorAna Kulić
AuthorJelena Marunica Karšaj
AuthorFilip Sedlić
Author's institution(Sestre milosrdnice University Hospital Center)
Scientific / art field,
discipline and subdiscipline		BIOMEDICINE AND HEALTHCARE
Clinical Medical Sciences
Abstract (english)
Background: Human induced pluripotent stem cells (hiPSCs) need to be thoroughly characterized to exploit their potential advantages in various aspects of biomedicine. The aim of this study was to compare the efficiency of cardiomyogenesis of two hiPSCs and two human embryonic stem cell (hESC) lines by genetic living cardiomyocyte labeling. We also analyzed the influence of spontaneous beating on cardiac differentiation. Methods: H1 and H9 hESC lines and C2a and C6a hiPSC lines were induced into in vitro directed cardiac differentiation. Cardiomyogenesis was evaluated by the analysis of cell cluster beating, cardiac protein expression by immunocytochemistry, ability of cells to generate calcium transients, and cardiomyocyte quantification by the myosin light chain 2v-enhanced green fluorescent protein gene construct delivered with a lentiviral vector. Results: Differentiation of all cell lines yielded spontaneously beating cell clusters, indicating the presence of functional cardiomyocytes. After the cell dissociation, H1-hESC-derived cardiomyocytes exhibited spontaneous calcium transients, corresponding to autonomous electrical activity and displayed ability to transmit them between the cells. Differentiated hESC and hiPSC cells exhibited striated sarcomeres and expressed cardiac proteins sarcomeric a-actinin and cardiac troponin T. Cardiomyocytes were the most abundant in differentiated H1 hESC line (20% more than in other tested lines). In all stem cell lines, cardiomyocyte enrichment was greater in beating than in non-beating cell clusters, irrespective of cardiomyogenesis efficiency. Conclusion: Although C2a and C6a hiPSC and H9 hESC lines exhibited efficient cardiomyogenesis, H1 hESC line yielded the greatest cardiomyocyte enrichment of all tested lines. Beating of cell clusters promotes cardiomyogenesis in tested hESCs and hiPSCs.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review
Publication versionPublished version
Journal titleThe International Journal of Developmental Biology
Numberingvol. 65, no. 10-11-12, pp. 537-543
p-ISSN0214-6282
e-ISSN1696-3547
DOIhttps://doi.org/10.1387/ijdb.210115fs
URN:NBNurn:nbn:hr:220:488644
Publication2021
Document URLhttps://ijdb.ehu.eus/article/210115fs
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Created on2024-09-26 12:35:10