prikaz prve stranice dokumenta Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation
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Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation
The International Journal of Developmental Biology, 65 (2021), 10-11-12; 537-543. https://doi.org/10.1387/ijdb.210115fs
Šepac, Ana; Bošnjak, Željko J.; Seiwerth, Sven; Sikirić, Sunčana; Regović Džombeta, Tihana; Kulić, Ana; Marunica Karšaj, Jelena; Sedlić, Filip

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Šepac, A., Bošnjak, Ž. J., Seiwerth, S., Sikirić, S., Regović Džombeta, T., Kulić, A. ... Sedlić, F. (2021). Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation. The International Journal of Developmental Biology, 65. (10-11-12), 537-543. doi: 10.1387/ijdb.210115fs

Šepac, Ana, et al. "Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation." The International Journal of Developmental Biology, vol. 65, br. 10-11-12, 2021, str. 537-543. https://doi.org/10.1387/ijdb.210115fs

Šepac, Ana, Željko J. Bošnjak, Sven Seiwerth, Sunčana Sikirić, Tihana Regović Džombeta, Ana Kulić, Jelena Marunica Karšaj i Filip Sedlić. "Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation." The International Journal of Developmental Biology 65, br. 10-11-12 (2021): 537-543. https://doi.org/10.1387/ijdb.210115fs

Šepac, A., et al. (2021) 'Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation', The International Journal of Developmental Biology, 65(10-11-12), str. 537-543. doi: 10.1387/ijdb.210115fs

Šepac A, Bošnjak ŽJ, Seiwerth S, Sikirić S, Regović Džombeta T, Kulić A, i sur.. Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation. The International Journal of Developmental Biology [Internet]. 2021. [pristupljeno 21.02.2025.];65(10-11-12):537-543. doi: 10.1387/ijdb.210115fs

A. Šepac, et al., "Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation", The International Journal of Developmental Biology, vol. 65, br. 10-11-12, str. 537-543, 2021. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:220:488644. [Citirano: 21.02.2025.]

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Naslov (engleski)Human C2a and C6a iPSC lines and H9 ESC line have less efficient cardiomyogenesis than H1 ESC line: Beating enhances cardiac differentiation
AutorAna Šepac
AutorŽeljko J. Bošnjak
AutorSven Seiwerth
AutorSunčana Sikirić
AutorTihana Regović Džombeta
AutorAna Kulić
AutorJelena Marunica Karšaj
AutorFilip Sedlić
Autorova ustanova(Klinički bolnički centar Sestre milosrdnice)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Sažetak (engleski)
Background: Human induced pluripotent stem cells (hiPSCs) need to be thoroughly characterized to exploit their potential advantages in various aspects of biomedicine. The aim of this study was to compare the efficiency of cardiomyogenesis of two hiPSCs and two human embryonic stem cell (hESC) lines by genetic living cardiomyocyte labeling. We also analyzed the influence of spontaneous beating on cardiac differentiation. Methods: H1 and H9 hESC lines and C2a and C6a hiPSC lines were induced into in vitro directed cardiac differentiation. Cardiomyogenesis was evaluated by the analysis of cell cluster beating, cardiac protein expression by immunocytochemistry, ability of cells to generate calcium transients, and cardiomyocyte quantification by the myosin light chain 2v-enhanced green fluorescent protein gene construct delivered with a lentiviral vector. Results: Differentiation of all cell lines yielded spontaneously beating cell clusters, indicating the presence of functional cardiomyocytes. After the cell dissociation, H1-hESC-derived cardiomyocytes exhibited spontaneous calcium transients, corresponding to autonomous electrical activity and displayed ability to transmit them between the cells. Differentiated hESC and hiPSC cells exhibited striated sarcomeres and expressed cardiac proteins sarcomeric a-actinin and cardiac troponin T. Cardiomyocytes were the most abundant in differentiated H1 hESC line (20% more than in other tested lines). In all stem cell lines, cardiomyocyte enrichment was greater in beating than in non-beating cell clusters, irrespective of cardiomyogenesis efficiency. Conclusion: Although C2a and C6a hiPSC and H9 hESC lines exhibited efficient cardiomyogenesis, H1 hESC line yielded the greatest cardiomyocyte enrichment of all tested lines. Beating of cell clusters promotes cardiomyogenesis in tested hESCs and hiPSCs.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaThe International Journal of Developmental Biology
Brojčani podacivol. 65, br. 10-11-12, str. 537-543
p-ISSN0214-6282
e-ISSN1696-3547
DOIhttps://doi.org/10.1387/ijdb.210115fs
URN:NBNurn:nbn:hr:220:488644
Datum objave publikacije2021
URL dokumentahttps://ijdb.ehu.eus/article/210115fs
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2024-09-26 12:35:10